![]() ![]() Aquat Toxicol 86:388–396Ĭostigan SL, Werner J, Ouellet JD, Hill LG, Law RD (2012) Expression profiling and gene ontology analysis in fathead minnow ( Pimephales promelas) liver following exposure to pulp and paper mill effluents. ![]() Mol Biol Evol 18:542–550Ĭhristianson-Heiska IL, Haavisto T, Paranko J, Bergelin E, Isomaa B (2008) Effects of wood extractives dehydroabietic acid and betulinol on reproductive physiology of zebrafish ( Danio rerio)-a two-generation study. Environ Toxicol Chem 27:1273–1278Ĭhiang EFL, Yan YL, Guiguen Y, Postlethwait J, Chung BC (2001) Two cyp19 (P450 aromatase) genes on duplicated zebrafish chromosomes are expressed in ovary or brain. J Toxicol Environ Health A 66:627–646Ĭarson J, Jenkins R, Wilson E, Howell W, Moore R (2008) Naturally occurring progesterone in loblolly pine ( Pinus taeda L.): a major steroid precursor of environmental androgens. Comp Biochem Physiol B 133:55–68īrinkworth LC, Hodson PV, Tabash S, Lee P (2003) CYP1A induction and blue sac disease in early developmental stages of rainbow trout ( Onchorynchus mykiss) exposed to retene. Environ Toxicol Chem 29:730–741īilliard SM, Hahn ME, Franks DG, Peterson RE, Bols NC, Hodson PV (2002) Binding of polycyclic aromatic hydrocarbons (PAHs) to teleost aryl hydrocarbon receptors (AHRs). Environ Toxicol Chem 17:68–87Īnkley GT, Bennett RS, Erickson RJ, Hoff DJ, Hornung MW, Johnson RD, Mount DR, Nichols JW, Russom CL, Schmieder PK, Serrrano JA, Tietge JE, Villeneuve DL (2010) Adverse outcome pathways: a conceptual framework to support ecotoxicology research and risk assessment. Bull Environ Contam Toxicol 16:270–274Īnkley G, Mihaich E, Stahl R, Tillitt D, Colborn T, McMaster S, Miller R, Bantle J, Campbell P, Denslow N, Dickerson R, Folmar L, Fry M, Giesy J, Gray LE, Guiney P, Hutchinson T, Kennedy S, Kramer V, LeBlanc G, Mayes M, Nimod A, Patino R, Peterson R, Purdy R, Ringer R, Thomas P, Touart L, Van Der Kraak G, Zacharewski T (1998) Overview of a workshop on screening methods for detecting potential (anti-) estrogenic/androgenic chemicals in wildlife. These results highlight the toxic potential of the pulp mill-contaminated sediment to aquatic biota, indicated by oestrogen-responsive cyp19a1b regulation in an immature fish ( 20dpfZF).Īhokas JT, Karki NT, Oikari A, Soivio A (1976) Mixed function mono-oxygenase of fish as an indicator of pollution of aquatic environments by industrial effluent. There was a significantly higher upregulation of cyp19a1b ( P = 0.001) by 0–5 cm compared to the that at the 20–30-cm layer at the 1-km site, indicating an oestrogenic impact of recent surface sediment near the mill compared to that in the site further downstream. While aromatase played a significant role, more specifically for both the sites 1 and 3 km downstream, the upper layers (0–5 cm) showed higher upregulation of cyp19a1b compared to the reference sediment located 9 km upstream from the mill. Changes in gene expression of cyp19a1b ( P < 0.001), but not of cyp1a and vtg1 as measured by qRT-PCR, suggested oestrogenic impact of the industrial contaminated sediment on the steroidogenic pathway of the fish. fischeri) bioluminescence by sediment elutriates indicated the non-toxic nature of the samples. Then, a whole-sediment bioassay with 20-day post-fertilization zebrafish ( 20dpfZF) to lake sediment (0–5-cm and 20–30-cm layers) was conducted for 3 days, and changes in cyp1a, cyp19a1b and vtg1transcript levels were assessed using quantitative reverse transcription PCR (qRT-PCR) analyses. Materials and methodsīioluminescence inhibition of Vibrio fischeri by sediment elutriates was measured to determine the overall toxicity of the sediment. This study examined the changes in transcript levels of key biomarkers, cytochrome P450 1A ( cyp1a), brain aromatase ( cyp19a1b) and vitellogenin 1 ( vtg1), in late larval zebrafish exposed to sediment cores from areas upstream and downstream (two distances) from a pulp and paper mill in Finland. Chemicals in effluents from the wood industry have been widely identified as sources of contamination affecting endocrine function in aquatic animals.
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